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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Cancer Prev Res (Phila). 2013 May 16;6(7):675–685. doi: 10.1158/1940-6207.CAPR-13-0064

Table 1.

Effect of NSAIDs on UV-induced PGE2 and Proliferation

Agent Dose (ppm) pg PGE2/μg Protein % of UV Control % BrdU positive cells % of UV control
no UV 404 ± 90a 21.6 2.4 ± 0.13a 15.7
UV 1872 ± 391 100.0 15.3 ± 0.96 100.0
Indo + UV 4 126 ± 37a 6.7 4.8 ± 0.64a 31.3
Celecoxib + UV 125 1716 ± 84 91.7 nd nd
Celecoxib + UV 250 818 ± 51a 43.7 nd nd
Celecoxib + UV 500 681 ± 125a 36.4 nd nd
Nap + UV 100 274 ± 84a 14.6 7.2 ± 1.48a 47.1
Nap + UV 200 168 ± 34a 9.0 nd nd
Nap + UV 400 114 ± 54a 6.1 6.7 ±1.36a 44.1
NO-Nap + UV 100 653 ± 57a 34.9 12.3 ± 2.24 80.4
NO-Nap + UV 200 440 ± 87a 23.5 nd nd
NO-Nap + UV 400 336 ± 53a 17.9 6.8 ± 2.23a 44.4
Asp + UV 60 1270 ± 106b 67.8 5.4 ± 2.06a 35.3
Asp + UV 250 701 ± 53a 37.4 nd nd
Asp + UV 750 337 ± 61a 18.0 8.1 ± 1.31a 52.6
NO-Asp + UV 108 1267 ± 118a 67.7 10.1 ±1.02a 66.0
NO-Asp + UV 450 232 ± 43a 12.4 nd nd
NO-Asp + UV 1350 162 ± 38a 8.7 9.7 ± 1.15a 63.3
Sul + UV 25 402 ± 196a 21.5 8.3 ± 0.31a 54.2
Sul + UV 75 174 ± 52a 9.3 8.4 ± 0.68a 54.9
Sul + UV 150 69 ± 20a 3.7 4.4 ± 0.70a 29.0

For PGE2 analysis, groups of 6-8 mice fed their respective experimental diets for 1 week were exposed to 220 mJ/cm2 UV (except the no UV group) and killed 6 hr later. The epidermis was chipped from frozen skin, PGE2 extracted and subjected to enzyme immunoassay as described under Methods and Materials. For BrdU labeling, groups of 3-4 mice fed their respective experimental diets for 1 week were exposed to 220 mJ/cm2 UV (except the no UV group) and killed 24 hrs later. All mice were injected with BrdU 1 hr prior to killing. Sections of skin were processed for immunohistochemical staining for BrdU. The number of BrdU positive basal cells and total number of basal cells per field were counted. The values represent the mean percentage of positive basal cells ± std. dev. The UV controls were statistically significantly different (p<0.01) from the no UV group. All other groups were compared to the UV only group. The statistical differences are shown by superscripts:

a

p<0.01

b

p<0.05.

nd = not determined.