Fig. 10.

CR8 attenuates spinal cord injury (SCI)-induced cell cycle activation at the injury site and in the lumbar dorsal horn. (a) CR8 attenuated SCI mediated increase in cyclin dependent kinase 4 (CDK4), cyclin E, and PCNA expression at 6 weeks after SCI. (b–d) Quantification of respective Western blots in panel a. (e) CR8 reduced SCI-induced increase in expression of cyclin D1. (f) Quantification of Western blot in (e). (g) Few cyclin D1⁺ cells were found in the dorsal horn of intact rat (g1). Cyclin D1⁺ cells increased 7 days after SCI (g2) and was attenuated in CR8-treated animals (g3). Scale bar = 100 μm. *p < 0.05 SCI groups vs sham groups; # p < 0.05 SCI CR8 vs SCI vehicle (Veh); n = 4–5/group. GAPDH = glyceraldehyde 3-phosphate dehydrogenase. PCNA proliferating cell nuclear antigen