Fig 2.

Fibrin-fibronectin protects B16F1 cells from TNFα. (A), PI⁺ B16F1 cells embedded in fibrin (Fib) or fibrin-fibronectin (FibFN) were counted in random optical field after treatment with TNFα (50 ng/ml) for 24 and 48 hours compared to vehicle. PI uptake in fibrin-embedded cells treated with vehicle is set to 1. (B), representative phase contrast microscopy images of B16F1 melanoma cells 96 hours after embedding in fibrin or fibrin-fibronectin (FibFN) and treatment with 50 ng/ml TNFα. Scale bar, 500 µm.