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. Author manuscript; available in PMC: 2013 Jul 5.
Published in final edited form as: Nat Cell Biol. 2010 Aug 1;12(9):853–862. doi: 10.1038/ncb2089

Figure 2. Developmental defects elicited upon K27 mutation are H3.3 specific.

Figure 2

a. Overexpression of H3.1 – GFP does not alter development or blastocyst formation. Zygotes were injected with mRNA for H3.1 – GFP wt as in Figure 1, cultured till they reached the blastocyst stage, fixed and analysed with the Cdx2 antibody. Confocal sections were taken every 2 μm. Shown are a middle section (left) and a full projection (right) of one representative blastocyst of 12 analysed. Scale bar is 50 μm.

b. Mutation of K27 in H3.1 does not alter developmental progression. Zygotes were microinjected as in figure 1a and their developmental stage was monitored daily. Scale bar is 50 μm.

c. Summary of development of embryos expressing H3.1 – GFP wt, K4R or K27R mutants of 5 independent experiments. Statistical analysis was performed using ANOVA.