Figure 3. Manumycin A corrects aberrant splicing of Clcn1 in HSA^LR DM1 model mice.

(a) RT-PCR analysis showed increased inclusion of Clcn1 exon 7A in HSA^LR mouse. (b) Quantification of the results shown in (a) (mean + SEM, n = 3). (c) RT-PCR analysis showed reduced inclusion of Clcn1 exon 7A 5 days after injection of manumycin A into TA muscles. (d) Quantification of the results shown in (a) (Mean + SEM, n = 3). The gel image was cropped around the region of interest and the samples (n = 3) were resolved in the same gel. Statistical significance was determined using t-tests (*p < 0.05, **p < 0.01).