Figure 4. The knockdown of H-Ras corrects aberrant splicing of Clcn1 in the presence of the expanded CUG repeat.

(a) Representative result of Western blot analysis of H-Ras in C2C12 cells. (b) Results of cellular splicing assays using Clcn1-L minigene, DM480 and siRNA. (c) Quantification of the results shown in (b) (mean + SEM, n = 3). The gel and blot image were cropped around the region of interest and the samples (n = 3) were resolved in the same gel or blot. Statistical significance was determined using t-tests (**p < 0.01).