Video 3. Simultaneous observation of ZAP70-EGFP recruitment and pMHC:TCR binding immediately after a living AND T cell lands on the SLB.

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DOI: 10.7554/eLife.00778.011

This video shows binding of pMHC:TCR, and is from the same cell as the ZAP70-EGFP data in Video 2. Agonist pMHC is labeled as MCC-Atto647N. These data were analyzed to create the spatial map of pMHC and ZAP70 positions displayed in Figure 4A.

DOI: http://dx.doi.org/10.7554/eLife.00778.011