Skip to main content
. Author manuscript; available in PMC: 2013 Jul 5.
Published in final edited form as: Am J Obstet Gynecol. 2009 May 8;201(1):70.e1–70.e9. doi: 10.1016/j.ajog.2009.02.010

FIGURE 7. Immunofluorescence of scFv H6 with USPC-4 cells and PBL analyzed by laser scanning confocal microscopy.

FIGURE 7

Representative micrographs of single-chain antibody fragment format (scFv) membrane staining (red) and phalloidin fluorescin isothiocyanate membrane staining (green) of unfixed cells in suspension. A, Labeling of uterine serous papillary carcinoma-4 (USPC-4) cells with scFv H6 and Alexa Fluor 568-labeled goat antimouse. B, Labeling of USPC-4 cells with phalloidin fluorescin isothiocyanate. C, Double immunofluorescence staining of USPC-4 with scFv H6 and phalloidin fluorescin isothiocyanate labeled. D, Double immunofluorescence staining of peripheral blood lymphocytes (PBL) with scFv H6 and phalloidin fluorescin isothiocyanate labeled.

Romani. Development of a human scFv against claudin-3. Am J Obstet Gynecol 2009.