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. Author manuscript; available in PMC: 2013 Jul 5.
Published in final edited form as: Cancer. 2011 Jun 20;117(24):5519–5528. doi: 10.1002/cncr.26215

Figure 3.

Figure 3

(Top) Representative knockdown of the Clostridium perfringens enterotoxin (CPE) receptors by anticlaudin-3 and anticlaudin-4 small interfering RNA (siRNA) in ovarian cancer stem cells (CSCs) is shown. The levels of claudin-3 and claudin-4 mRNA in OVA-1R-CSC CD44+ cells transfected with claudin-3 and claudin-4 siRNA duplexes (gray bars) were analyzed using quantitative real-time polymerase chain reaction and expressed as percentage of control cells (black bars). (Bottom) Representative dose-dependent CPE-mediated cytotoxicity of OVA-1R-CSCs transfected with claudin-3/-4 siRNA duplexes after 1 hour exposure to 0.5 μg/mL (black bars) and 1 μg/mL (gray bars) of CPE (P = .004) is shown. Mock-transfected cells and untransfected cancer stem cells were used as controls. Results are presented as percentage of viable cells when compared with number of untreated control cells (ie, 100% viable cells).