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. Author manuscript; available in PMC: 2013 Oct 11.
Published in final edited form as: Cell. 2013 Apr 4;153(2):449–460. doi: 10.1016/j.cell.2013.03.020

Fig. 7. p53 activity in HSCs promotes an anti-tumor microenvironment.

Fig. 7

(A) Quantification of viable Cd45+Cd11b+Gr1− cells in CCl4-treated mice (n = 3 from each genotype).

(B and C) Quantitative RT-PCR of FACS-sorted Cd45+Cd11b+Gr1− cells for Il1β (B) and Mrc1 (C) expression. The results are normalized to the average expression of the housekeeping genes Hprt and Ubc and relative to the expression in HSC-p53Δ/Δ mice. Values are mean + SD of 3 independent livers per genotype.

(D-H) Staining for Cd11b (red) and Il1β (green) (D), and Iba1 (red) and Mrc1 (green) (E), revealing less Cd11b+ or Iba1+ macrophages, less Cd11b+Il1β+ macrophages and more Iba1+Mrc1+ macrophages in HSC-p53Δ/Δ CCl4-treated livers than in control livers. Quantification of Cd11b+ cells (% of total DAPI+ cells) (F), Cd11b+Il1β+ cells (% of total Cd11b+ cells) (G), and Mrc1+Iba1+ cells (% of total Iba+ cells) (H). Scale bars represent 100 μm. Yellow arrows indicate double-stained cells.

See also Figure S7.