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. Author manuscript; available in PMC: 2013 Jul 5.
Published in final edited form as: J Huntingtons Dis. 2012 Nov 27;1(2):221–241. doi: 10.3233/JHD-129005

Fig. 9.

Fig. 9

Mutant Htt expression is similar in FVB.CAG140 and YAC128 mice. Western blot of soluble mHtt protein from a half-brain homogenate (A) Two different anti-huntingtin antibodies demonstrate that there are similar levels of mHtt protein in YAC128 and FVB.CAG140 mice. (B) Levels are normalized to calnexin and then to endogenous Htt levels. The expression is also very similar between 6 and 12 months of age. As expected, FVB.CAG140 HET and HOM mice have reduced expression of WT Htt compared to YAC128 mice. Filter retardation-based assay to quantify the presence of insoluble mHtt protein (C) The amount of insoluble protein detected by the EM48 antibody is also similar between the two strains of mice, however the levels of insoluble protein are much higher at 12 months than 6 months. Data are mean ± SEM; N = 3 for all groups in the western blot, and 2 for all groups in the filter retardation assay.