Table 1.
Plasmids used in this study.
| Plasmid | Description | Reference |
|---|---|---|
| pJRS525 | broad host range cloning vector; aad9 (Spr) | (McIver & Scott, 1997) |
| pProExHTb | expression vector with N-terminal 6x His; bla (Apr) | Invitrogen |
| pKSM357 | his6-ptsI; bla (Apr) | this study |
| pKSM637 | luc under PtrxT in pJRS525; aad9 (Spr) | (Gold, 2011) |
| pBlue∆ptsI | ∆ptsI in pBluescriptII KS-; bla (Apr) | this study |
| pKSM645 | ∆ptsI with non-polar aad9 (Spr) | this study |
| pKSM712 | his6-ptsH; bla (Apr) | (Hondorp et al., 2012) |
| pMga1-His | WT mga1 (SF370) with C-terminal His6; bla (Apr) | (Hondorp et al., 2012) |
| pKSM801 | WT mga4 (GA40634) with C-terminal His6; bla (Apr) | (Hondorp et al., 2012) |
| pKSM807 | WT mga1 under native Pmga1; aad9 (Spr) | (Hondorp et al., 2012) |
| pKSM808 | WT mga4-his6 (GA40634) under native Pmga4; aad9 (Spr) | (Hondorp et al., 2012) |
| pKSM809 | WT mga1-his6 under native Pmga1; aad9 (Spr) | (Hondorp et al., 2012) |
| pKSM819 | ∆29mga4-his6 under PrpsL; aad9 (Spr) | (Hondorp et al., 2012) |
| pKSM822 | ∆29mga4-his6 under native Pmga4; aad9 (Spr) | (Hondorp et al., 2012) |
| pKSM824 | H324A mga4 with C-terminal His6; bla (Apr) | this study |
| pKSM825 | H324D mga4 with C-terminal His6; bla (Apr) | this study |
| pKSM832 | H324A mga4-his6 under native Pmga4; aad9 (Spr) | this study |
| pKSM833 | H324D mga4-his6 under native Pmga4; aad9 (Spr) | this study |
| pKSM836 | H204A mga1 with C-terminal His6; bla (Apr) | this study |
| pKSM837 | H204D mga1 with C-terminal His6; bla (Apr) | this study |
| pKSM838 | H270A mga1 with C-terminal His6; bla (Apr) | this study |
| pKSM839 | H270D mga1 with C-terminal His6; bla (Apr) | this study |
| pKSM841 | H204A/H270A mga1 with C-terminal His6; bla (Apr) | this study |
| pKSM842 | H204D/H270D mga1 with C-terminal His6; bla (Apr) | this study |
| pKSM849 | H204A/H270A mga1-his6 under native Pmga1; aad9 (Spr) | this study |
| pKSM850 | H204D/H270D mga1-his6 under native Pmga1; aad9 (Spr) | this study |
| pKSM857 | H204D mga4 with C-terminal His6; bla (Apr) | this study |
| pKSM858 | H270A mga4 with C-terminal His6; bla (Apr) | this study |
| pKSM859 | H270D mga4 with C-terminal His6; bla (Apr) | this study |
| pKSM860 | H204A mga4 with C-terminal His6; bla (Apr) | this study |
| pKSM861 | H204A/H270A mga4 with C-terminal His6; bla (Apr) | this study |
| pKSM862 | H204D/H270D mga4 with C-terminal His6; bla (Apr) | this study |
| pKSM863 | H204A/H270A/H324A mga4 with C-terminal His6; bla (Apr) | this study |
| pKSM864 | H204D/H270D/H324D mga4 with C-terminal His6; bla (Apr) | this study |
| pKSM865 | H204A mga4-his6 under native Pmga4; aad9 (Spr) | this study |
| pKSM866 | H204D mga4-his6 under native Pmga4; aad9 (Spr) | this study |
| pKSM867 | H270A mga4-his6 under native Pmga4; aad9 (Spr) | this study |
| pKSM868 | H270D mga4-his6 under native Pmga4; aad9 (Spr) | this study |
| pKSM869 | H204A/H270A mga4-his6 under native Pmga4; aad9 (Spr) | this study |
| pKSM870 | H204D/H270D mga4-his6 under native Pmga4; aad9 (Spr) | this study |
| pKSM871 | H204A/H270A/H324A mga4-his6 under native Pmga4; aad9 (Spr) | this study |
| pKSM872 | H204D/H270D/H324D mga4-his6 under native Pmga4; aad9 (Spr) | this study |
| pKSM876 | D/D ∆29mga4-his6 under native Pmga4; aad9 (Spr) | this study |
| pKSM877 | A/A ∆29mga4-his6 under constitutive PrpsL; aad9 (Spr) | this study |
| pKSM879 | his6-pepck; bla (Apr) | this study |