Figure 5. Pargyline treatment reduced subcutaneous glioma xenograft tumor growth and induced apoptosis in vivo.

(A) NOD/SCID mice were subcutaneously implanted with 1 × 10⁶ U87 cells. After tumors reached a measurable size, mice were treated daily with vehicle or pargyline (100 mg/kg/ bodyweight) for a period of 30 days. Tumor size was measured with calipers every 5 days. A representative picture of a tumor is shown as an inset. (B) Body weights of both vehicle- and pargyline-treated mice were measured weekly. Column, mean body weights. (C) Ki-67 staining in control- and pargyline-treated tumors. Representative images are depicted (left panel). * P <0.05. Ki-67 labelling was quantified as the mean Ki-67 percentage based on at least 10 randomly selected high-power microscope fields per group (right panel) (D) TUNEL staining for apoptosis in control- and pargyline-treated tumors. Representative images are depicted (left panel). TUNEL labelling was quantified as the mean TUNEL labelling percentage based on at least 10 randomly selected high-power microscope fields per group (right panel).