Figure 1. High-throughput RNAi screen to identify vimentin expression regulating siRNAs.

A: The silencing efficacy of the vimentin targeting siRNA used as a positive control as well as the specificity of the vimentin antibody used in the LMA analysis, was confirmed in MDA-MB-231(SA) cells with qRT-PCR and Western blotting methods. B: LMA slides were stained for total protein (Sypro Ruby, yellow) and for vimentin expression (vimentin, green). Lysate spots derived from vimentin targeting siRNA (indicated with arrows) show decrease in vimentin but not total protein expression. C: A correlation plot (vimentin signal Z-scores) of the two replicate RNAi screens in MDA-MB-231(SA) cells.