Table 1.

Bacterial genomes and sequence read lengths used in the GAGE-B evaluation

Species	Genome size (Mb)	GC content (%)	Sequencing technology	Read length (bp)	Fragment length (bp)	Coverage
A.hydrophila SSU	4.7	65	HiSeq	101	180	250×
B.cereus VD118	5.4	35	HiSeq	101	180	100–300×
B.cereus ATCC 10987	5.4	35	MiSeq	250	600	100×
B.fragilis HMW 615	5.3	43	HiSeq	101	180	250×
M.abscessus 6G-0125-R	5.1	64	HiSeq	100	335	115×
M.abscessus 6G-0125-R	5.1	64	MiSeq	250	335a	100×
R.sphaeroides 2.4.1	4.6	69	HiSeq	101	220	210×
R.sphaeroides 2.4.1	4.6	69	MiSeq	251	540	100×
S.aureus M0927	2.9	33	HiSeq	101	180	250×
V.cholerae CO1032(5)	4.0	48	HiSeq	100	335	110×
V.cholerae CO1032(5)	4.0	48	MiSeq	250	335a	100×
X.axonopodis pv. Manihotis UA323	2.9	33	HiSeq	101	400	250×

Note: All datasets used paired-end reads from both ends of every fragment.

^aThe fragment lengths for two of the MiSeq libraries were relatively short, only 335 bp, because the same library was used for both HiSeq and MiSeq sequencing of those species.