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. 2013 Jun 13;4(6):e671. doi: 10.1038/cddis.2013.202

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Copyright © 2013 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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NFAT5 inhibits binding between β-catenin and p300/CBP. (a) HEK293 cells were transfected with HA-CBP, Myc-NFAT5, or both, and after 48 h, lysate was collected and incubated with the GST-tagged β-catenin protein. After several washings, bound proteins were eluted and an anti-HA western blot assay was performed. (b) HEK293 cells were transfected with Flag-β-catenin, Myc-NFAT5, or both, and after 48 h, lysate was collected and incubated with the GST-tagged CH3 domain of p300. After several washings, bound proteins were eluted and an anti-Flag western blot assay was performed. (c) HEK293 cells were transfected with HA-tagged CBP and Flag-tagged β-catenin, with or without Myc-NFAT5 as indicated. Anti-HA immunoprecipitation was performed on the cell lysate, and bound proteins were analyzed via anti-Flag western blot. Whole-cell lysate was analyzed as a control.