Figure 5.

miR-101 acts as a tumor suppressor and induces senescence in glioma cell lines. (a) The inhibition of proliferation by miR-101 in glioma cells. (b) A wound-healing assay analyzes the effect of miR-101 on glioma cells. *P<0.05. (c) The effect of miR-101 on senescence in glioma cells was measured with SA-β-gal staining. Arrows point to the green senescent cells. *P<0.05. (d) Transmission electron micrographs of the morphology of the glioma cells transfected with miR-101 mimics. The U251 cells exhibited cytoplasmic and nuclear concentration, chromatin aggregation at the periphery of the nucleus and mitochondrial vacuolar degeneration after transfection with miR-101. Two different lesions were observed in the treated U87 cells: one was cellular edema, including in the cytoplasm, nucleus and mitochondria; the other was cellular condensation, including cytoplasmic, nuclear and mitochondrial vacuolar degeneration