Figure 1.

Validation of microarray data by RT-qPCR methodology. (a) The numbers of overlapping genes modulated in response to hyperosmotic treatments are shown in a Venn diagram. ↑ represents upregulated genes; ↓ represents downregulated genes. (b and c) Comparison of gene expression data obtained from microarray analysis (gray column; mean of duplicates±S.E.M.) and from RT-qPCR analysis performed on samples used for microarray analysis (hashed columns), and on samples obtained from four additional independent experiments (black columns; mean±S.E.M., n=4). The results are expressed as fold stimulation of treated cells over control