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Cyclins and cdks expression during hyperosmotic stress. (a–e) ARPE-19 cells were submitted to 4- or 24-h stimulation without (Ct) or with 100 mM NaCl (Na100) or 200 mM sucrose (Su200). Total protein expression of cyclins D1, B1, A1, E1 and β-actin (a); cdk1, cdk2, cdk4 and β-actin (b); p21, p27 and β-actin (c); CDC6, PCNA and β-actin (d); were determined by western blot analysis
