Table 3. Lack of hyperosmotic stress on ARPE-19 cell death.
N | EA | LA | NC | |
---|---|---|---|---|
Control | 95.13±0.38 | 0.04±0.02 | 0.11±0.02 | 4.71±0.37 |
Na100 | 90.88±2.70 | 0.02±0.01 | 0.05±0.04 | 9.05±2.67 |
Su200 | 89.14±5.02 | 0.04±0.01 | 0.13±0.05 | 10.69±4.98 |
ARPE-19 cells were submitted to 24-h stimulation without (Control) or with 100 mM NaCl (Na100) or 200 mM sucrose (Su200). Cells stained with propidium iodide and annexin IV were analyzed by FACS. Results are expressed as normal (N), early apoptotic (EA), late apoptotic (LA) or necrotic (NC) cells (in percent of total), and are the mean±S.E.M. (n=3). Data were analyzed using repeated-measures ANOVA and Tukey's multiple comparison tests, P>0.05