Figure 4.

HY-PDT induces apoptosis in HepG2 cells. The cells were treated with 0.1, 0.2, 0.5 and 1 μg/ml HY for 8 h and irradiated for 5 min. The rate of apoptosis of treated cells was determined 18 h post incubation. The percentage of apoptotic cells was determined by FACSCanto II and analyzed using a FCAP array (BD Biosciences). The apoptotic rate of HepG2 cells was concentration dependent after HY-PDT exposure. Data are mean±S.E.M. of n=3 independent experiments carried out in triplicates. Statistically significant differences are labeled as *P<0.05, **P<0.01 and ***P<0.001, compared with untreated cells using Student's unpaired two-tailed t-test