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. 2013 Jun 27;69(Pt 7):723–729. doi: 10.1107/S1744309113015182

Figure 3.

Figure 3

(a) SDS–PAGE gel with results from the microtubule co-sedimentation assay. The ladder is labeled in kDa. Lanes 2–5 are supernatant samples; lanes 6–9 are pellet samples. In the following, the amount of protein loaded into each gel lane is given in parentheses. Lane 1, Mast-M1 (5.0 mg); lanes 2 and 6, MAPF incubated with microtubules (1.6 mg MAPF + 1.0 mg microtubules); lanes 3 and 7, Mast-M1 not incubated with microtubules (0.5 mg); lanes 4 and 8, Mast-M1 incubated with microtubules (0.5 mg Mast-M1 + 1.0 mg microtubules); lanes 5 and 9, Mast-M1 incubated with microtubules (0.2 mg Mast-M1 + 1.0 mg microtubules). (b) Gel-filtration graph of tubulin (red line), Mast-M1 (blue line) and Mast-M1 incubated with tubulin (green line).