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Purification of Robo1Fn23SeMet. (a) Superdex 200 26/60 size-exclusion chromatography elution profile of Robo1Fn23SeMet. The protein peak elutes at a volume of 217 ml, corresponding to ∼30 kDa, which is close to the calculated weight of the protein (26 kDa). (b) SDS–PAGE analysis of (a). Lane M contains molecular-mass markers (labelled in kDa).
