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. Author manuscript; available in PMC: 2013 Jul 5.
Published in final edited form as: Biochem J. 2009 Nov 11;424(2):191–200. doi: 10.1042/BJ20090730

Figure 3. The amino acid sequence and structure features of the full-length Aedes cadherin protein.

Figure 3

(A) Five overlapping cadherin cDNA fragments, G31, G7, C13, G10 and C3 were amplified from an Aedes midgut cDNA library or whole Aedes cDNA samples and these fragments were assembled by five sequential steps of subcloning. This Aedes cDNA contains a complete ORF, which encodes a full-length Aedes cadherin protein. The protein contains a signal peptide (SIG), 11 cadherin repeats (CR1–11), a membrane-proximal region (MPR), a transmembrane domain (TM) and a cytoplasmic domain (CD). Toxin-binding regions were localized in CR8–11. (B) The putative N-terminal signal peptide is underlined with dots and the transmembrane domain is double underlined. The 11 cadherin repeat sequences are in bold. Putative calcium-binding sites are underlined and the integrin recognition sequence RGD (aa 1098–1101) is in italics and underlined. The ATP/GTP-binding site is dash-underlined and the predicted N-glycosylated residues (Asn) are labelled with an asterisk above the letter N.