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. Author manuscript; available in PMC: 2013 Jul 5.
Published in final edited form as: Biochem J. 2009 Nov 11;424(2):191–200. doi: 10.1042/BJ20090730

Figure 4. In toxin overlay assays and ELISA, the Cry11Aa toxin binds cadherin fragments and cadherin repeats.

Figure 4

(A) Partial cDNA fragments, G7, C13 and G10 (90 pmol each) were separated by SDS/PAGE (8 % gel) and stained using Coomassie Blue (lanes 1–3). These fragments were electotransferred to a PVDF membrane, which was incubated with 20 nM Cry11Aa toxin. Unbound toxin was removed by washing, the membrane was incubated with anti-Cry11Aa antiserum (1:2000) and then visualized by luminol (lanes 5–7). (B) The purified cadherin repeats CR7–11 (30 pmol each) were resolved by SDS/PAGE (15 % gel) and stained using Coomassie Blue (lanes 1–5). (C) The cadherin repeats, CR8 (○), CR9 (■), CR10 (□) and CR11 (▲) show dose-dependent binding to immobilized Cry11Aa (0.4 μg), but CR7 (●) does not bind Cry11Aa. OD405, A 405.