Figure 4.

The serum proteome analysis of a mouse model with autoimmune hepatitis by 2-DE and a targeted iTRAQ validation on clinical serum. The pooled serum samples were depleted of albumin and IgG using ProteoExtract Albumin Removal Kit, and protein fractions were used for the subsequent differential expression analysis. Serum protein expression profile of AIH mice was compared with normal mouse serum by 2-DE, differential proteins were identified by MS/MS. On the other hand, the AIH patient’s serum was pretreated and labeled with iTRAQ reagents to perform LC-MS/MS, and the target proteins were selected to validate their expression levels in AIH patient’s sera by iTRAQ quantification.