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Extraction of mitochondrial membrane proteins by the SMA copolymer. (A) BN-PAGE analysis of proteins extracted from isolated mitochondria following incubation with increasing concentrations of SMA copolymer (lanes 1 to 5) or DDM (lanes 6 to 10). Arrows indicate locations of detectable bands. (B) Immunodetection of Sdh3 (left panel, lanes 1–5) and Tim50 (right panel, lanes 6–10) extracted from mitochondria by SMA and DDM at the concentrations indicated.
