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Effects of population size and criterion used in LH sampling on the optimization. Increase in the number of mutants captured during independent DE runs for population sizes N = 19 (green lines) and N = 27 (blue lines). Pink line: Model performance for the initial guess for the parameter vector (O = 72 hits; Omax = 119). Red line: WT viability in glucose is not enforced during LH sampling phase.
