Table 8.

Synthetic lethality induced by elimination of phosphorylation/dephosphorylation reactions

Eliminated reaction	Impacted single mutation strains that are
	viable (inviable) before (after) perturbation
Whi5 phosphorylationby Bck2	cln3 Δ, Multicopy BCK2, cdh1 Δ, sic1 Δ, swi5 Δ,
	CLB5-db Δ, net1-ts, GAL-CLB2, APC-A
CKI phosphorylation by Cln2	bck2 Δ, GAL-SIC1, net1-ts, APC-A
CKI phosphorylation by Clb2	GAL-CLN3, cdh1 Δ, GAL-CLB5, CLB1 clb2 Δ
CKI dephosphorylation by Cdc14	bck2 Δ, cdh1 Δ, GAL-CLB2, APC-A
Whi5 phosphorylation by Cln3	bck2 Δ, cdh1 Δ, APC-A
SBF phosphorylation by Clb2	cdh1 Δ, CLB5-db Δ, APC-A
Whi5 phosphorylation by Cln2	bck2 Δ,APC-A
Whi5 dephosphorylation by Cdc14	APC-A
Net1 dephosphorylation by PPX	Multicopy CDC15

Upon setting phosphorylation/dephosphorylation rate constants to zero (left column), viability is lost in several single mutation strains (right column).