Table 1.
Parameters for the strains harboring the improved pathways identified through directed evolution
| Wild-type | R1 | R2 | |
|---|---|---|---|
| Specific Growth Rate |
0.0694 ± 0.003 |
0.0919 ± 0.006 |
0.102 ± 0.002 |
| (h-1 ) |
|
|
|
| Cellobiose Consumption |
1.78 ± 0.06 |
2.33 ± 0.02 |
2.65 ± 0.02 |
| (g cellobiose/(L · h)) |
|
|
|
| Ethanol Productivity |
0.611 ± 0.02 |
0.815 ± 0.03 |
1.00 ± 0.03 |
| (g ethanol/(L · h)) |
|
|
|
| Yield |
|
|
|
| (g ethanol / g cellobiose) | 0.4073 ± 0.02 | 0.4236 ± 0.0008 | 0.4363 ± 0.004 |
*Values determined at time point when >95% cellobiose was consumed for the strain.
Errors are standard deviations of biological triplicates from three independent experiments. Fermentation conditions are in 8% cellobiose rich media and oxygen-limited conditions.