Table 2.

Specific activity measured from the crude lysate of the engineered β-glucosidase

	p-NGP	Cellobiose
	(U/mg protein)	(U/mg protein)
Control	0.00729 ± 0.0001	0.0021 ± 0.002
Wild-type	0.193 ± 0.01	0.0668 ± 0.001
L173H	0.130 ± 0.01	0.124 ± 0.004
H23L	0.219 ± 0.004	0.0738 ± 0.006
L173H + H23L	0.156 ± 0.007	0.170 ± 0.01

One unit is defined as one μmol/min and the control is the lysate from a strain with no heterologously expressed β-glucosidase. Errors are derived from the standard deviation of biological triplicates.