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. 2013 Jul 5;8(7):e67591. doi: 10.1371/journal.pone.0067591

Figure 4. Knockdown of CKS2 inhibits TPC-1 cell growth.

Figure 4

A. Effect of miR-26a transfection or CKS2 knockdown on TPC-1 cell proliferation measured by CCK8 assay. B. Colony formation assay of TPC-1 cells transfected with miR-26a mimic, miR control, CKS2 siRNA and siRNA control, and the relative numbers of colonies counted under a microscope in five vision fields. Error bars correspond to 95% confidence intervals. C. Cell-cycle distribution of TPC-1 cells transfected with miR-26a mimic, miR control, CKS2 siRNA and siRNA control for 48 h. D. Flow cytometry and annexin V assays reflect the number of apoptotic TPC-1 cells after transfection with miR-26a mimic, miR control, CKS2 siRNA or siRNA control.