Skip to main content
. 2013 Jul 5;8(7):e67591. doi: 10.1371/journal.pone.0067591

Figure 6. CKS2 overexpression rescues the growth suppressive effect of miR-26a.

Figure 6

A. Effects of CKS2 over-expression on miR-26a-TPC-1 cell proliferation. MiR-26a+CKS2 = miR-26a-TPC-1 cell transfected with CKS2-PMCB vector; MiR-26a +EV = miR-26a-TPC-1 cell transfected with EV-PMCB vector. B. Colony formation assay of miR-26a-TPC-1 cells transfected with CKS2-PMCB or EV-PMCB, and the relative numbers of colonies counted under microscope in five vision fields. Error bars correspond to 95% confidence intervals. C. Cell-cycle distribution of miR-26a-TPC-1 cells transfected with CKS2-PMCB or EV-PMCB for 48 h. D. Flow cytometry and Annexin V assays reflect the number of apoptotic miR-26a-TPC-1 cells after transfection with CKS2-PMCB, or EV-PMCB.