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. 2013 Jul 5;8(7):e66752. doi: 10.1371/journal.pone.0066752

Figure 1. Expression of Enpp1 in breast cancer cell lines.

Figure 1

Enpp1 (A, B) mRNA and (C) protein expression was determined in (A) NT2.5 murine breast cancer cells and bone sublines, and (B, C) immortalized normal human mammary epithelial cell lines and human breast cancer cell lines by (A, B) qRT-PCR and (C) Western analysis. (A, B) Data are representative of three independent experiments performed in triplicate and expressed as the mean ± s.e.m (**p<0.01, ***p<0.001). (C) Protein expression was determined by Western analysis performed on equal amounts of protein from total cell lysates.