Table 1. Affinities KD (μM) of protein ligands to different Parkin proteins.
Protein/Ligand | UblD1 | UbcH72 | Ubq3 |
FL4-FLAG-Parkin RT | 5.4 | 4.7 | 82 |
FL4-FLAG-Parkin 56°C | 7.4 | 8.1 | n/a |
R0RBR Domain RT | 6 | 7.1 | 96 |
Ubiquitin-like domain of Parkin; 2Ubiquitin conjugating enzyme E2; 3Ubiquitin; 4 Full-length.