Table 1. Strains and plasmids used in this study.
Strains or plasmids | Genotype/Description | Sources |
Strains | ||
E. coli BL21 star(DE3) | F– ompT hsdSB (rB – mB –) gal dcm rne131 (DE3) | Invitrogen |
E. coli BL21 star(DE3) ΔxylAB | Knockout of xylA and xylB encoding xylose isomerase and xylulose kinase | This study |
Plasmids | ||
pACYCduet-1 | Cmr oriP15A lacIq T7p | Novagen |
pA-xdh | pACYCduet-1 harboring C. crescentus xdh | This study |
pA-xylC | pACYCduet-1 harboring C. crescentus xylC | This study |
pA-xdhxylC | pACYCduet-1 harboring C. crescentus xdh and xylC | This study |
pKD46 | Apr oriR101 repA(Ts) λ Red (γ, β and exo) | Coli Genetic Stock Center |
pKD4 | FRT-Kanr-FRT oriR6K | Coli Genetic Stock Center |
pCP20 | Apr Cmr repA(Ts) FLP | Coli Genetic Stock Center |