Table 1. Identification of Differentially Expressed protein spots.
Spot no.a | Accession no. | Protein description | theoretical(pI/kDa) | database | Matched peptidesb | Unmatched peptidesc | Coverage (%) | E- values | Protein scroed | Peptides identifiede |
20 | gi|195963353 | 3-hydroxyisobutyrate dehydrogenase [Bombyx mori] | 9.14/34.02 | Inv_others Px_EST | 1 | 6 | 4 | 5.6e-08 | 66 | R.SPIPLGAVATQLYR.I |
21 | gi|237636932 | 14-3-3 zeta [Heliothis virescens] | 4.84/28.1 | NCBInr | 8 | 24 | 36 | 4.4e-43 | 472 | R.NTVVEDSQKAYQDAFEISK.S |
12 | gi|357618425 | putative cxpwmw03 [Danaus plexippus] | 7.71/34.74 | NCBInr | 4 | 13 | 8 | 3.5e-14 | 168 | R.AAVDAGFVPNDLQIGQTGK.I |
15 | gi|270298186 | Enolase [Pieris rapae] | 5.58/47.12 | Inv_others Px_EST | 12 | 23 | 62 | 3.5e-65 | 583 | K.FGLDSTAVGDEGGFAPNIQNNK.E |
26 | gi|3582502 | glutathione S-transferase isozyme 3 [Plutella xylostella] | 8.5/24.1 | Px_EST | 2 | 7 | 6 | 1.1e-15 | 176 | R.AVTFLIFTEGLK.K |
17 | gi|85165 | tropomyosin, exon 9B - fruit fly [Drosophila melanogaster] | 4.67/32.9 | NCBInr | 3 | 8 | 5 | 8.8e-13 | 159 | K.ALQNAESEVAALNRR.I |
16 | gi|284927832 | arginine kinase-like protein [Plutella xylostella] | 5.76/39.66 | Inv_others Px_EST | 4 | 16 | 6 | 1.4e-38 | 378 | R.LGFLTFCPTNLGTTVR.A |
28 | gi|301508512 | apolipophorin-III [Plutella xylostella] | 7.91/18.4 | Px_EST | 4 | 12 | 8 | 3.5e-23 | 262 | R.EAPAGSTQLQDLEK.H |
6 | gi|53148459 | tropomyosin I [Plutella xylostella] | 4.74/32.54 | NCBInr | 1 | 14 | 3 | 7e-08 | 79 | K.LLEAQQSADENNR.M |
13 | gi|357614862 | acyl-CoA dehydrogenase [Danaus plexippus] | 7.51/48.5 | NCBInr | 4 | 4 | 6 | 2.2e-25 | 261 | K.IYQIYEGTSQIQR.L |
18 | gi|22450121 | glyceraldehyde-3-phosphate dehydrogenase [Plutella xylostella] | 6.54/35.47 | Px_EST | 5 | 11 | 18 | 4.4e-27 | 395 | K.LISWYDNEYGYSNR.V |
19 | gi|357607952 | fructose 1,6-bisphosphate aldolase [Danaus plexippus] | 8.09/42.03 | NCBInr | 2 | 12 | 6 | 5.6e-09 | 115 | R.IVPIVEPEVLPDGEHDLDR.A |
23 | gi|300470333 | glutathione S-transferase delta [Plutella xylostella] | 6.32/23.92 | Px_EST | 12 | 7 | 36 | 7e-66 | 524 | R.FGDYFYPQLFGGAPEDKEK.L |
5 | gi|112982822 | phosphoglyceromutase [Bombyx mori] | 6.33/28.6 | NCBInr | 2 | 8 | 5 | 4.5e-10 | 125 | K.AEGYQFDVAHTSVLKR.A |
22 | gi|49532926 | Glutathione S transferase 2-like protein [Plutella xylostella] | 5.85/23.53 | Px_EST | 4 | 7 | 12 | 4.6e-25 | 295 | R.RPDLDQQYPGFAK.V |
8 | gi|328670887 | voltage-dependent anion-selective channel [Helicoverpa armigera] | 6.96/30.1 | NCBInr | 2 | 7 | 8 | 3.5e-15 | 197 | K.YAVKDYGLTFTEK.W |
31 | gi|209978476 | Chemosensory protein [Plutella xylostella] | 6.88/15.45 | NCBInr | 2 | 16 | 4 | 5.6e-12 | 135 | K.CVLDQGKCSPDG.K |
Spot No. is the unique number of the position where the spot display in the master gel;
The number of peaks that match the trypsin peptides;
The number of peaks that do not match the trypsin peptides;
Protein score based on combined mass/mass spectrums;
Each spot corresponding to a certain protein had at least one of the shown peptides identified.