Table 4. Morphological classification of male scorpions with Discriminant Function Analysis (DFA) to show correctly classified versus misclassified groups.

Males
	Predicted group membership
Actual group membership	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	Total Actual Groups
1. Brownsville	6	2	0	0	1	0	1	2	0	0	0	0	0	0	0	0	1	13
2. Tamapulian	1	11	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	13
3. Cen TX (E)	0	0	4	0	2	0	2	0	0	2	0	0	0	1	0	0	0	11
4. Coahuila	0	0	1	44	0	0	0	1	0	1	0	1	0	0	0	0	0	48
5. CO (E)	3	1	6	1	15	1	0	1	0	3	0	1	0	1	2	1	0	36
6. NE-N (M)	2	0	0	0	0	5	1	0	0	0	0	2	0	1	2	0	0	13
7. NE (M)	4	0	5	1	3	1	23	1	0	0	0	6	0	2	2	0	0	48
8. Laredo (K)	5	0	2	4	3	1	1	21	1	0	0	3	3	3	0	0	0	47
9. Chinati (G)	0	0	0	0	0	0	0	0	7	0	0	0	0	0	0	0	0	7
10. Ag Sp (I)	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	4
11. Oliver Lee (D)	0	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0	0	4
12. Big Piney (M)	2	0	1	0	3	3	4	1	1	0	0	16	0	7	0	0	0	38
13. Falcon Lake (K)	0	0	0	1	0	0	0	1	0	0	0	0	8	0	0	0	0	10
14. OK (M)	0	0	2	0	0	1	1	0	0	0	0	2	0	10	0	0	0	16
15. Villanueva (E)	0	0	0	0	0	0	0	0	0	0	0	0	0	0	9	0	0	9
16. Bbend 1 (F)	0	0	0	1	0	0	0	0	1	0	0	0	0	1	0	12	8	23
17. BBend 2 (F)	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	15	16
Total Predicted Groups	23	14	21	52	27	12	33	28	10	10	4	32	11	26	15	14	24	356

Each row represents actual groups whereas the columns represent predicted groups. Groups identified through the TCS analysis are shown in parentheses. The reduction in classification error (57.6%) shows the accuracy of the DFA compared to random classification. See Protocol S1 for further information concerning population designations and statistical analyses.