Table 1. Cell densities and positive controls used in reporter cell stimulations.

Cell line	Cell density	Positive control
THP-1 MD2-CD14	1*106 cells/ml	E.coli K12 lipopolysaccharide Ultrapure (LPS, 100 ng/ml)
THP-1 DefMyD	2*106 cells/ml	L-Ala-gamma-D-Glu-mDAP (TriDAP, 10 µg/ml)
HEK-Blue TLR2	2.8*105 cells/ml	Pam2CGDPKHPKSF (FSL-1, 1 µg/ml)
HEK-Blue TLR3	2.8*105 cells/ml	Poly(I:C) high molecular weight (HMW, 1 mg/ml)
HEK-Blue TLR4	1.4*106 cells/ml	E.coli K12 lipopolysaccharide Ultrapure (LPS, 100 ng/ml)
HEK-Blue TLR5	1.4*106 cells/ml	Recombinant flagellin from S.typhimurium (Rec-FLA-ST,100 ng/ml)
HEK-Blue TLR7	2.2*106 cells/ml	Imiquimod (5 µg/ml)
HEK-Blue TLR8	2.2*106 cells/ml	Single stranded RNA (ssRNA40/LyoVec™, 5 µg/ml)
HEK-Blue TLR9	4.5*106 cells/ml	Type B CpG oligonucleotide (ODN 2006, 100 mg/ml)
HEK-Blue NOD1	2.8*106 cells/ml	L-Ala-gamma-D-Glu-mDAP (TriDAP, 10 µg/ml)
HEK-Blue NOD2	1.4*106 cells/ml	MurNac-L-Ala-gamma-D-Glu-mDAP (M-TriDAP, 10 µg/ml)