Table 3. Candidates of novel miRNAs from R. glutinosa.
miRNA ID | Precursor gene ID | Precursor length (nt) | Reads | Sequences | Energy(kcal mol−1) | qRT-PCRidentification | ||
FP | SP | |||||||
Z2 | Unigene12916_All | 144 | 6 | 0 | ACAUUAGGACAAAUUAUCACG | −51.6 | No | |
rgl-miR7797a | Unigene151_All | 141 | 46 | 56 | AAGACGGAAUCAAACCUCAA | −48.2 | Yes | |
rgl-miR7797b | Unigene45997_All | 126 | 0 | 33 | UUUGAUUUCGUCUUACAUUUUUC | −19.37 | Yes | |
Z5 | Unigene20830_All | 84 | 13 | 0 | AGAGGAGUGUACCAUCACCGCC | −44.3 | No | |
rgl-miR7798 | Unigene21450_All | 229 | 9 | 16 | AGGGAGUGUUUGCAAAAACU | −67.1 | Yes | |
Z8 | Unigene26960_All | 200 | 208 | 106 | UUGUCAGGCUUGUUAUUCUCC | −68.6 | No | |
Z9 | Unigene30417_All | 272 | 27 | 27 | CGAGUAGCGCUAGAGGUCCAACA | −66.86 | No | |
Z10 | Unigene30676_All | 107 | 58 | 0 | AUCCAUGGGGAGAGGAAGCACA | −43.52 | No | |
Z11 | Unigene32348_All | 72 | 81 | 0 | CUUUUAUAAAGCUGUCGGGACA | −27.4 | No | |
rgl-miR7799 | Unigene34159_All | 125 | 7 | 0 | AGUGGAAUAGGAGAUCUCAA | −44.63 | Yes | |
Z14 | Unigene40078_All | 91 | 17 | 14 | AGGGGAAGGAUUUCAAAUGAC | −38.6 | No | |
Z16 | Unigene40998_All | 92 | 6 | 0 | AGAGGGAGUACUAUUGAAGGA | −36 | No | |
rgl-miR7800 | Unigene51345_All | 95 | 21 | 0 | UAUUUUUGUGUCGUUAUGGUC | −35.2 | Yes | |
rgl-miR7801 | Unigene51544_All | 128 | 18 | 0 | UACGAGAUGAAACACAGUUUG | −38.2 | Yes | |
rgl-miR7802 | Unigene51775_All | 106 | 6 | 0 | AGGGAGUGUUUGCAAUCACUAAA | −28.17 | Yes | |
rgl-miR7803a | Unigene51839_All | 152 | 10 | 0 | UACGGAUAAUUGACACGUGUAUA | −62.4 | Yes | |
rgl-miR7803b-5p | Unigene84940_All | 117 | 8 | 0 | GGAUGAUUGCCACGUGUAUA | −56.8 | Yes | |
rgl-miR7803b-3p | 5 | 0 | UACACGUGUCAAUCAUCUAU | Yes | ||||
Z24 | Unigene52051_All | 113 | 135 | 0 | GGCGAACUGCUCGAGCUGCU | −35.9 | No | |
rgl-miR7804-5p | Unigene55578_All | 160 | 24 | 0 | AGGGGUGUUCAUCGAAUCGAAUU | −42.7 | Yes | |
rgl-miR7804-3p | 6 | 0 | UUUAAUCGAAUGAACAUUUUAAA | No | ||||
Z27 | Unigene57696_All | 97 | 18 | 0 | ACCGUUGAUGGUAUCAAAAUC | −47.4 | No | |
rgl-miR7805-5p | Unigene64556_All | 69 | 12 | 0 | AAAUUUGGUGUAGUGAAUAGU | −38.7 | Yes | |
rgl-miR7805-3p | 7 | 0 | UAUUCAUUUACACCAAAUUUGG | No | ||||
Z33 | Unigene73437_All | 184 | 109 | 50 | GUAGCAUCAUCAAGAUUCACA | −68.3 | No | |
rgl-miR7806 | Unigene74190_All | 126 | 593 | 526 | UAGAAGAUGUCCACAUGAGCA | −38.27 | Yes | |
rgl-miR7807a-5p | Unigene82131_All | 138 | 9 | 0 | AACUAUAUGAAAAUCUCAAUU | −41.4 | Yes | |
rgl-miR7807a-3p | 64 | 24 | UUGGGAUUUGCAUACAGUUAC | Yes | ||||
rgl-miR7807b-5p | Unigene40448_All | 144 | 8 | 0 | UAACUAUAUGAAAAUCUCAAUU | −59.2 | Yes | |
rgl-miR7807b-5p | 13 | 0 | UUGAGAUUUUCAUAUAGUUACU | Yes | ||||
Z38-5p | Unigene87020_All | 117 | 162 | 0 | ACUCGCAUCUACAAGAACAUA | −49.02 | No | |
Z38-3p | 69 | 0 | UGUUCUUGUAAAUGGGUAGUUA | No | ||||
rgl-miR7808 | Unigene891_All | 315 | 26 | 0 | AAGGAUGCUCGAUUCAGAAGAA | −76.69 | Yes | |
Z40 | Unigene891_All | 248 | 9 | 0 | UUCUUCUGAAUCGAGCAUCCUU | −42.14 | No | |
C03 | Unigene17031_All | 168 | 0 | 8 | CAACUCUGGAUAUUGAUUCCUCA | −30.45 | No | |
rgl-miR7809 | Unigene18424_All | 115 | 0 | 108 | UCCCAUUGCAUCAGCGGACACA | −31.82 | Yes | |
C05 | Unigene21450_All | 229 | 0 | 10 | AGGGAGUGUUUGCAAAAACU | −67.1 | No | |
rgl-miR7810 | Unigene26764_All | 91 | 0 | 5 | AGAGGAAGAGUUUUCUGGCUC | −26 | Yes | |
rgl-miR7811 | Unigene41058_All | 86 | 0 | 369 | UGAAUGGAGAUACGGAAUGAAGC | −22 | Yes | |
rgl-miR7972 | Unigene30260_All | 207 | 0 | 10 | UUGUCAGGCUUGUUAUUCUCC | −53.71 | Yes | |
C21 | Unigene49775_All | 90 | 0 | 9 | AGAGGUCUGAGGUUCGAUUUUCA | −22 | No |