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. 2013 Jul 5;8(7):e68498. doi: 10.1371/journal.pone.0068498

Figure 2. Fluorescence HPLC determination of the level of formation of O2 – in response to an increase in intraluminal pressure.

Figure 2

The elevation in intraluminal pressure from 40 mm Hg to 120 mm Hg induced a significantly increased concentration of 2-OH-E+, the oxidation product of the superoxide detecting probe DHE by O2 –, normalized to tissue protein (n = 4 separate experiments for each group, *denote P<0.05 compared to control level of 2-OH-E+ to that following the increase in intraluminal pressure to 120 mm Hg.