FIGURE 5.

Akt-2 but not Akt-1 phosphorylates Foxo1 and Foxo3a downstream of Cbl-b. Naïve CD4⁺CD25⁻ T cells from WT, Akt-1^−/−, and Akt-2^−/− (A), or WT, Cbl-b^−/−, Akt-1^−/−, Cbl-b^−/−Akt-1^−/−, Akt-2^−/−, and Cbl-b^−/−Akt-2^−/− mice (B) were stimulated with anti-CD3 for times indicated and lysed. The cell lysates were blotted with phospho-Abs against Foxo1 and Foxo3a, and reprobed with anti-actin. The presence or absence of Akt-1, Akt-2, and Cbl-b was determined by immunoblotting with anti-Akt-1, anti-Akt-2, and anti-Cbl-b, respectively. The data shown are one representative of three independent experiments.