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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Cancer Res. 2013 Apr 18;73(13):3987–3996. doi: 10.1158/0008-5472.CAN-12-3859

Figure 4.

Figure 4

Promotion of pancreatic cancer cell migration and invasion by overexpression of FOXM1 isoforms. Untransfected COLO357 cells (control) and COLO357 cells transfected with an empty pcDNA3.1 vector (control) or FOXM1a, FOXM1b, or FOXM1c expression vectors were used. A, cell scratch-wound assay. B, cell migration assay. C, cell invasion assay. Assay details are provided in Supplementary Materials and Methods. Note: increased expression of FOXM1b and FOXM1c but not FOXM1a substantially promoted the horizontal and vertical migration and invasion of tumor cells. *P < 0.01 (Student t-test). D. Western blot analyses. FoxM1c upregulated the expression of Cyclin D1, MMP-2, MMP-9, uPA, uPAR, VEGF, and c-myc, but downregulated the expression of p27 and p21.