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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: J Immunol. 2013 Jun 10;191(2):848–856. doi: 10.4049/jimmunol.1202941

Figure 5.

Figure 5

Alveolitis induced by instillation of complement-activated serum. (A) C5a/C5a des Arg levels in DBP+/+ and DBP-/- mouse serum activated with CVF as measured by ELISA (n = 3). (B) Total Gr-1+ neutrophils in the BAL of DBP+/+ and DBP-/- mice 4 hours after instillation of 50 μl of 50% dilution (in PBS) of +/+ or -/- C-activated serum (n = 5). (C) Total leukocytes in the BAL from DBP+/+ and DBP-/- mice measured 4 hours after instillation of 50 μl of 50% dilution (in PBS) of +/+ or -/- heat-inactivated (56°C for 45 min) CVF-treated serum (n = 4). Numbers represent mean ± SEM, statistical significance is indicated, NS = not significant. (D) In vitro under-agarose chemotaxis assay using bone-marrow derived neutrophils from DBP+/+ and -/- mice. Numbers represent the net (buffer control subtracted) migration distance of cells toward either DBP+/+ or -/- complement-activated serum (n = 12). DBP-/- cells migrated significantly less (P < 0.01) toward DBP-/- complement-activated serum than all other groups.