Figure 6. Alterations in HSC/MPP subsets in Hoxa9−/−Flt3l−/− mice.

A) Synergistic reduction in LSK⁺ CD150⁻ Flt3⁺ cells in Hoxa9−/−Flt3l−/− mice. LSK⁺ cells were visualized based on differential expression of CD150 and Flt3 then fractionated by Flt3 density into Flt3⁻, Flt3^lo, and Flt3⁺ subsets (boxed regions, left to right, respectively). The boundaries of the boxed regions were determined based on loss of CD150 expression which accompanies acquisition of Flt3. B) Increase in LSK⁺ CD150⁺ CD34^−lo progenitors in Hoxa9−/−Flt3l−/− mice. C) Increased percentage of CD41⁺ LSK⁺ CD150⁺ CD34^−lo myeloid-biased cells in Hoxa9−/−Flt3l−/− mice. D) Summary of Flt3⁻, Flt3^lo, and Flt3⁺ LSK⁺ subsets in the four genotypes. E) Summary of LSK⁺ CD150⁺ CD34^−lo progenitors in the four genotypes. F) Absolute number of LSK⁺ CD150⁺ CD34^−lo cells per 4 hind limb leg bones per genotype. G) Summary of percentages of CD41⁺ cells within the LSK⁺ CD150⁺ CD34^−lo gate. Bar graphs reflect the mean ± SD of 3–5 mice per genotype.