Figure 4. p38α signaling in CD103⁺ MLN DCs drives iTreg differentiation independently of IL-27, IL-6 and IL-12.

(A) Expression of IL-27 in CD103⁺ MLN DCs isolated from WT and p38α^ΔDC mice. (B) Expression of Foxp3 in OT-II T cells activated with WT or p38α-deficient CD103⁺ DCs, in the presence of OVA (0.05 μg/ml), with or without αIL-27for 5 days. (C) Foxp3 expression in OT-II T cells activated with CD103⁺ MLN DCs isolated from WT, p38α^ΔDC, Il6^–/–, p38α^ΔDC Il6^–/–, Il12a^–/–, or p38α^ΔDC Il12a^–/– mice, in the presence of OVA (0.05 μg/ml), for 5 days. Data are representative of 2 independent experiments (n=3 mice per group).