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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: J Cell Physiol. 2013 Jul;228(7):1536–1550. doi: 10.1002/jcp.24312

Figure 4. FGF23 RNA levels are not regulated by Pi but the peptide synergistically enhances late gene expression.

Figure 4

(A) FGF23 levels in response to a time course of Pi treatment as measured by microarray. (B) The lack of FGF23 response to elevated Pi was confirmed by qRT-PCR in MC3T3-E1 cells treated for indicated times. C-fos is shown as a positive control. (C) Cells were serum starved overnight and treated with FGF23 (500ng/ml) with Klotho (500ng/ml) and Heparin (5ug) with or without Pi as indicated for 60 minutes and RNA analyzed by qRT-PCR. Results are calculated as fold increase relative to the non-treated control for each pair. (D) Cells were treated for 24 hours in growth medium with FGF23 and Pi as in (C) and RNA analyzed by qRT-PCR. Results were calculated as in (C) for each pair. (n=3) *p<0.01 compared to control (1mM), #p<0.01 compared to Pi-stimulated (student T test).