Table I.
Isolation of recombinant cecropinXJ based on 1 l of bacterial culture.
| Purification step | Total protein (g) | TrxA-cecropinXJ (g) | Purity (%)c |
|---|---|---|---|
| Sonicated supernatant | 1.147±0.614a | 0.998±0.215b | Not applicable |
| Ammonium sulfate precipitation | 0.715±0.347a | 0.429±0.208b | >60 |
| HisTrap | 0.016±0.003a | 0.015±0.002c | >90 |
The wet weight of cells was 4.754±0.681 g.
a
Protein concentration was determined by Bradford protein assay.
b
Percentage of fusion protein (TrxA-cecropinXJ) from total proteins was estimated by Tricine-SDS-PAGE gel scanning.
c
Purity of protein was estimated by staining Tricine-SDS-PAGE gel with Coomassie blue.