Figure 3.

Effect of EA on PCNA-positive cells in cerebral I/R-injured rats. Cerebral tissues from each group (n=5) were processed for an immunohistochemical assay. The nuclei of all cells were visualized by hematoxylin staining and the PCNA-positive cells were stained red with AP-red solution. The PCNA-positive cells were counted at four randomly selected microscopic fields at ×400 magnification. PCNA-positive cell rate was expressed as the ratio of red-stained cells to the blue-stained total cells. Data are averages with SE (error bars). ^*P<0.05, vs. SC group; ^#P<0.05, vs. IC group. SC, sham-operated control; IC, ischemic control; EA, electroacupuncture; I/R, ischemia/reperfusion; PCNA, proliferating cell nuclear antigen; AP, alkaline phosphatase.