Figure 4.

Effect of EA on the cyclin D1-positive cell rate in cerebral I/R-injured rats. Cerebral tissues from each group (n=5) were processed for an immunohistochemical assay. The nuclei of all cells were visualized by hematoxylin staining and the cyclin D1-positive cells were stained sepia with DAB solution. Cyclin D1-positive cells were counted at four randomly selected microscopic fields at ×400 magnification. The cyclin D1-positive cell rate was expressed as the ratio of sepia-stained cells to the blue-stained total cells. Data are averages with SE (error bars). ^*P<0.05, vs. SC group; ^#P<0.05, vs. IC group. SC, sham-operated control; IC, ischemic control; EA, electroacu-puncture; I/R, ischemia/reperfusion; DAB, diaminobenzidine.